Application Notes for
Centrifugal Partition Chromatography
LiLiChro
Explore real purification examples using centrifugal partition chromatography from pharmaceutical compounds and ionisable lipids to natural extracts, food-grade materials, and method development studies.
Looking for a scalable alternative to solid-phase chromatography?
Explore LiLiChro’s liquid-liquid chromatography (LLC) application notes. Discover how centrifugal partition chromatography (CPC) can support compound purification, molecule separation, natural product isolation, cannabinoid purification, and pharma R&D workflows without a solid stationary phase. Download our application notes and share it with your research team.
If you want to know more about our method, then visit our technology site where we share the main principles of liquid-liquid chromatography and centrifugal partition chromatography.
Application Notes
Short teaser:
Cyclosporine pre-purification with CPC: see how support-free liquid-liquid chromatography can help separate crude cyclosporine fractions before final polishing, with controlled recovery of apolar impurities, the main product fraction, and polar post-fractions.
Best for:
Pharmaceutical R&D teams, API purification specialists, Purification scientists, Process development teams, CPC and liquid-liquid chromatography users, Labs evaluating alternatives to preparative HPLC or flash chromatography
Short teaser:
See how CPC can remove chlorophyll, alkaloids, and other organically soluble compounds from crude vegetable leaf extracts — helping produce cleaner water-soluble protein fractions through scalable liquid-liquid chromatography.
Best for:
Plant protein purification teams, Food and nutraceutical R&D, Natural product purification specialists, Bioprocess and downstream processing teams, Labs working with crude vegetable leaf extracts, Teams evaluating CPC for chlorophyll removal or pigment remediation
Short teaser:
Explore how CPC can support ionisable lipid purification under water-free conditions. This application note highlights a liquid-liquid chromatography workflow for separating hydrophobic lipid compounds where conventional aqueous systems may not be suitable.
Best for:
Lipid nanoparticle and drug delivery R&D teams, Pharmaceutical purification scientists, Biotech process development teams, Ionisable lipid and hydrophobic compound specialists, Labs working with water-sensitive purification workflows, Teams evaluating CPC for pharmaceutical compound purification
Short teaser:
See how CPC can support fast purification of flavoring materials from complex biological samples using support-free liquid-liquid chromatography — a practical workflow for isolating valuable aroma or flavor compounds from challenging natural matrices.
Best for:
Flavor and fragrance R&D teams, Natural product purification specialists, Food and beverage ingredient developers, Biological sample preparation teams, Nutraceutical and botanical extract researchers, Labs evaluating CPC for complex natural matrices
Short teaser:
See how miniLiLi performs in a column loading test using methyl and ethyl paraben. This application note supports liquid-liquid chromatography method development by showing how sample loading affects separation performance, resolution, and practical workflow setup.
Best for:
CPC method development teams, Analytical and preparative chromatography labs, Purification scientists, R&D teams evaluating miniLiLi, Labs planning scale-up from miniLiLi to larger CPC systems, Teams testing sample loading and separation performance
Short teaser:
See how CPC can support EGCG purification from green tea polyphenol extract using support-free liquid-liquid chromatography — a practical workflow for isolating high-value natural compounds from complex botanical matrices.
Best for:
Natural product purification teams, Nutraceutical R&D specialists, Botanical extract researchers, Food-grade compound purification teams, Polyphenol and antioxidant researchers, Labs evaluating CPC for plant extract purification
Short teaser:
Cyclosporine pre-purification with CPC: see how support-free liquid-liquid chromatography can help separate crude cyclosporine fractions before final polishing, with controlled recovery of apolar impurities, the main product fraction, and polar post-fractions.
Best for:
Pharmaceutical R&D teams, API purification specialists, Purification scientists, Process development teams, CPC and liquid-liquid chromatography users, Labs evaluating alternatives to preparative HPLC or flash chromatography
Short teaser:
Explore how CPC can support ionisable lipid purification under water-free conditions. This application note highlights a liquid-liquid chromatography workflow for separating hydrophobic lipid compounds where conventional aqueous systems may not be suitable.
Best for:
Lipid nanoparticle and drug delivery R&D teams, Pharmaceutical purification scientists, Biotech process development teams, Ionisable lipid and hydrophobic compound specialists, Labs working with water-sensitive purification workflows, Teams evaluating CPC for pharmaceutical compound purification
Short teaser:
See how CPC can remove chlorophyll, alkaloids, and other organically soluble compounds from crude vegetable leaf extracts — helping produce cleaner water-soluble protein fractions through scalable liquid-liquid chromatography.
Best for:
Plant protein purification teams, Food and nutraceutical R&D, Natural product purification specialists, Bioprocess and downstream processing teams, Labs working with crude vegetable leaf extracts, Teams evaluating CPC for chlorophyll removal or pigment remediation
Short teaser:
See how CPC can support fast purification of flavoring materials from complex biological samples using support-free liquid-liquid chromatography — a practical workflow for isolating valuable aroma or flavor compounds from challenging natural matrices.
Best for:
Flavor and fragrance R&D teams, Natural product purification specialists, Food and beverage ingredient developers, Biological sample preparation teams, Nutraceutical and botanical extract researchers, Labs evaluating CPC for complex natural matrices
Short teaser:
See how CPC can support EGCG purification from green tea polyphenol extract using support-free liquid-liquid chromatography — a practical workflow for isolating high-value natural compounds from complex botanical matrices.
Best for:
Natural product purification teams, Nutraceutical R&D specialists, Botanical extract researchers, Food-grade compound purification teams, Polyphenol and antioxidant researchers, Labs evaluating CPC for plant extract purification
Short teaser:
See how miniLiLi performs in a column loading test using methyl and ethyl paraben. This application note supports liquid-liquid chromatography method development by showing how sample loading affects separation performance, resolution, and practical workflow setup.
Best for:
CPC method development teams, Analytical and preparative chromatography labs, Purification scientists, R&D teams evaluating miniLiLi, Labs planning scale-up from miniLiLi to larger CPC systems, Teams testing sample loading and separation performance