miniLiLi Column Loading Test Using Methyl and Ethyl Paraben
Column loading is an important part of method development, especially when a separation needs to move from analytical testing toward practical purification. This application note uses a methyl and ethyl paraben mixture to evaluate miniLiLi performance in a controlled liquid-liquid chromatography loading test.
The note is useful for understanding how miniLiLi can support early-stage CPC method development, sample loading evaluation, and separation optimization before scale-up. Download the full application note to review the test setup, loading conditions, chromatographic behavior, and practical implications for method transfer.
Relevant for:
Method description:
Equipment: miniLiLi 35 ml column
Mode: Descending mode
Solvent system: 135ml hexane; 15ml EtOAc; 60ml acetonitrile; 240ml water
Flow rate: 1 ml/min
Rotor speed: 1500 rpm
Sample: 10mg, 25mg, 100mg paraben mix solved in EtOAc (0.05, 0.05, and 0.25ml)
Detection: fraction by HPLC
Fraction: 1.8ml fraction, start 6 min
Download the full application note
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