Technology

The Liquid-Liquid chromatography

Liquid-liquid chromatography is an advanced technology that effectively combines extraction and chromatography as a common solution. Because it uses a liquid stationary phase, liquid-liquid chromatography offers many advantages over traditional liquid-solid chromatographic separation techniques. These include high sample loading capacity and the possibility of lossless sample recovery.

Advantage of liquid-liquid technology

Stationary phase regeneration
Scale up (column shapes, silica gel and solvents – so everything – are different)
Time consuming and expensive development:

• Silica gel tests from different manufacturers
• Run to run solvent system optimalisation

Both phases are liquid, simple distillation
Simple multiplication:

• Loading is same
• n [1/min] relies on r [m]
• Linear scale up
Short development time

• Find an optimal biphasic solvent system. Vial test by HPLC or TLC – 1-3 day
• Finetuning with miniLiLi – 1 week

LiLiCHRO devices do this in a highly efficient way

They can be used for a very large number of target components and can be optimised for a wide range of different operating modes. It can be used as a basis for laboratory technologies and scientific and academic research and applications that can extend the application of these devices far beyond the laboratory. To this end, LiLiCHRO is committed to working with its partners.

What are the advantages of having a LiLiCHRO device?

LiLiCHRO’s devices provide one of the best value for money solutions to various cleaning and separation problems.

The LLC, in a unique way in chromatography, is very easy to scale up (unlike prepLC). On several occasions we faced problems where we had very little amount of material available.

With the smallest device available for Laboratory, 1-2 g of sample is needed for a feasibility study.
The LiLiCHRO, and the world’s smallest instrument, works with a miniLiLi 20 ml cell volume and a flow rate of 1-2 ml/min, and is therefore often comparable to a conventional analytical HPLC. This is a big step forward for LLC.

Our prepLLC device, LiLiCHRO prep, on the other hand, has a cell volume of 100 litres – five times larger than the largest unit ever created. The completely redesigned cell system has also greatly increased efficiency, raising the monthly purifiable volume to 0.5-2 T.

The perpetual development of the LiLiCHRO

Industrial new cells

The reinvented cell design has increased efficiency by an order of magnitude compared to the old cells.

Industrial new rotor

Due to the redesigned rotor structure, the pressure drop in the device has been greatly reduced, so the pressure drop of 50-100 bar that was previously experienced, is only 10-30 bar on the new rotor.

Industrial new rotary-union

The constant need for seal replacement has been eliminated.

Industrial new mixer-settler

Two-phase production is always a challenge on an industrial scale. A two-phase system must first be mixed and then separated in order to start the separation process. At a flow rate of 20 l/min we had to rethink this process as well. We are proud that the new system is capable of feeding the unit continuously and safely.

Want to talk to LiLiCHRO?