Chlorophyll Removal from Vegetable Extracts

Crude aqueous vegetable leaf extracts often contain a complex mixture of proteins, alkaloids, terpenes, and pigments such as chlorophyll. This application note shows how centrifugal partition chromatography (CPC) can support chlorophyll remediation by separating unwanted organically soluble compounds from water-soluble protein fractions.

The workflow uses LiLiChro’s midiLiLi equipment with a water-based buffer / water-saturated MTBE solvent system, UV detection at 280 nm, and ascending mode operation. The result demonstrates selective removal of chlorophyll and alkaloids, producing cleaner protein fractions suitable for downstream processing in both R&D and manufacturing environments.

Relevant for:

Plant protein purification teams, Food and nutraceutical R&D, Natural product purification specialists, Bioprocess and downstream processing teams, Labs working with crude vegetable leaf extracts, Teams evaluating CPC for chlorophyll removal or pigment remediation

Method description:

Equipment: midiLiLi 135ml column

Mode: Ascending mode

Solvent system: Water based Buffer / Water saturated MTBA

Flow rate: 14 ml/min

Rotor speed: 300-800 rpm

Sample: Crude aqueous vegetable leaf extracts

Detection: 280 nm UV

Fraction: Fractions collected at predefined intervals (see chromatogram)

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