Ionisable Lipid Purification with CPC Under Water-Free Conditions
Ionisable lipids are important in advanced pharmaceutical and biotech workflows, but their hydrophobic character can make purification challenging — especially when water-free conditions are required. This application note demonstrates how centrifugal partition chromatography (CPC) can be applied to ionisable lipid purification using a support-free liquid-liquid chromatography approach.
The workflow shows how CPC can offer an alternative separation strategy for sensitive, hydrophobic compounds where traditional solid-phase chromatography or aqueous methods may create limitations. Download the full application note to review the method concept, separation setup, and purification result.
Relevant for:
Method description:
Equipment: miniLiLi equipment (34ml column volume)
Mode: Descending mode, Hexane:MTBE:Acetonitrile:AcOH 200:60:140:2 system, mixed, equlibrated and separated.
MiniLiLi equipment was uploaded with upper phase at DSC mode, 4 ml/min flowrate, 300 1/min rotation speed.
After uploading the rotor was stopped, 150 mg ionisable lipid (88% content by QNMR) was diluted by 200 μl hexane and injected and washed twice 2×200 μl upper phase.
After injection rotor speed raised to 1200 1/min and started the lower phase at 2 ml/min flowrate.
Fraction collected by UV-signal at 210nm.
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