Cyclosporine purification using liquid-liquid chromatography
Cyclosporine purification can be challenging when crude material contains closely related impurities that require selective fractionation before final polishing. This application note presents a liquid-liquid chromatography workflow using LiLiChro’s miniLiLi CPC equipment for cyclosporine pre-purification.
The method demonstrates how centrifugal partition chromatography can support pharmaceutical purification workflows through support-free separation, UV-based fraction collection, and recovery-focused processing. Download the full application note to review the method setup, solvent system, chromatogram, fraction strategy, and purification outcome.
Relevant for:
Method description:
Equipment: miniLiLi equipment (35ml column volume)
Mode: Ascending mode with extrusion
Stationary phase: Acetonitrile / Water;
Ratio: 45:55
Moving phase: Hexane / MTBE / Acetonitrile; Ratio: 65:35:5
Phases weren’t equilibrated.
MiniLiLi equipment was uploaded with stationary phase flowrate, 300 1/min rotation speed.
After uploading injection 0.2 ml cyclosporine solution.
After injection rotor speed raised to 1000 1/min and started the moving phase at 2 ml/min flowrate.
Fraction collected by UV-signal at 210 nm. After cyclosporine peaks some polar isomers collected by extrusion….
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