Fast, Flavoring Material Purification from Biological Sample
Flavoring materials from biological samples often come from complex natural matrices, where target compounds may be present alongside pigments, lipids, proteins, and other interfering components. This application note presents a fast liquid-liquid chromatography workflow for purifying flavoring-related compounds from a biological sample.
Using centrifugal partition chromatography (CPC), the method demonstrates how support-free separation can help isolate valuable compounds from natural sources without relying on a solid stationary phase. Download the full application note to review the workflow, separation approach, and purification result.
Relevant for:
Method description:
Equipment: miniLiLi 35 ml column
Mode: Ascending mode
Solvent system: Water:AcN:Hexane 10:40:50
Pressure: 11 bar
Flow rate: 1 ml/min
Rotor speed: 1500 rpm
Sample: 10mg, 25mg, 100mg paraben mix solved in EtOAc (0.05, 0.05, and 0.25ml)
Pre-fraction and post-fraction were taken for 1,5 minutes (6 ml volume each) in brown vials. The main fraction was taken for 6 minutes (24 ml volume each) in brown vials. Samples were kept in the refrigerator and were not deoxygenated.
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