food-grade separation

Fast, Flavoring Material Purification from Biological Sample

Flavoring materials from biological samples often come from complex natural matrices, where target compounds may be present alongside pigments, lipids, proteins, and other interfering components. This application note presents a fast liquid-liquid chromatography workflow for purifying flavoring-related compounds from a biological sample.

Using centrifugal partition chromatography (CPC), the method demonstrates how support-free separation can help isolate valuable compounds from natural sources without relying on a solid stationary phase. Download the full application note to review the workflow, separation approach, and purification result.

Relevant for:

Flavor and fragrance R&D teams, Natural product purification specialists, Food and beverage ingredient developers, Biological sample preparation teams, Nutraceutical and botanical extract researchers, Labs evaluating CPC for complex natural matrices

Method description:

Equipment: miniLiLi 35 ml column

Mode: Ascending mode

Solvent system: Water:AcN:Hexane 10:40:50

Pressure: 11 bar

Flow rate: 1 ml/min

Rotor speed: 1500 rpm

Sample: 10mg, 25mg, 100mg paraben mix solved in EtOAc (0.05, 0.05, and 0.25ml)

Pre-fraction and post-fraction were taken for 1,5 minutes (6 ml volume each) in brown vials. The main fraction was taken for 6 minutes (24 ml volume each) in brown vials. Samples were kept in the refrigerator and were not deoxygenated.

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