EGCG Purification from Green Tea Polyphenol Extract

EGCG, or epigallocatechin gallate, is a high-value green tea polyphenol with strong relevance in nutraceutical, botanical, and natural product research. This application note presents a liquid-liquid chromatography workflow for EGCG purification from green tea polyphenol extract using centrifugal partition chromatography (CPC).

The note demonstrates how support-free separation can help isolate target polyphenols from complex plant-derived matrices where conventional solid-phase chromatography may face limitations. Download the full application note to review the method setup, solvent system, separation workflow, and purification outcome.

Relevant for:

Natural product purification teams, Nutraceutical R&D specialists, Botanical extract researchers, Food-grade compound purification teams, Polyphenol and antioxidant researchers, Labs evaluating CPC for plant extract purification

Method description:

Equipment: miniLiLi 35 ml column

Mode: Ascending mode

Solvent system: MTBE / Water, ratio 1:1

Flow rate: 1 ml/min

Rotor speed: 1000 rpm

Sample: 50 mg crude EGCG / 0.25 ml EtOAc

Detection: 280 nm UV

Fraction: 7 ml fraction volume, collection starts after 7 min

Download the full application note

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