Liquid-Liquid Chromatography for Scalable Compound Purification

LiLiCHRO

Discover the new generation of preparative chromatography that rethinks traditional extraction and purification workflows.

We provide efficient preparative separation

In the sector of:

Pharmaceuticals
Cannabis
Biotech
Natural Extracts
Food & Nutraceuticals
Cosmetics
Veterany Pharma

For:

How efficient?

Highly Efficient preparative chromatographic solutions

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See how CPC improves ROI

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Why LiLiChro?

Inexpensive solvents

Plug&Play system
Easy maintenance

Low pressure = long lasting
quality parts

High yield and throughput

Return on investment measured in weeks instead of years

No expensive silica columns

Advantages of Centrifugal Partition Chromatography

When is CPC worth considering?

1. When recovery matters
CPC can reduce the risk of irreversible adsorption because there is no solid stationary
phase.

2. When crude or complex samples are difficult to purify
Useful for natural extracts, cannabinoids, fermentation-derived compounds,
intermediates, peptides, lipids, alkaloids, and other complex matrices.

3. When column cost or column fouling becomes a bottleneck
CPC avoids recurring solid stationary phase replacement.

4. When scale-up is part of the project
CPC can support method development from lab-scale toward pilot and industrial
purification.

5. When solvent strategy and sustainability matter
CPC can support solvent-system optimization and solvent recycling in suitable workflows.

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Explore the Technology Behind LiLiChro

If you want to know more about our method, then visit our technology site where we share the main principles of liquid-liquid chromatography and centrifugal partition chromatography.

We can help you identify your purification bottleneck

Top Questions about Centrifugal Partition Chromatoraphy

What are the most common applications of CPC?

CPC is well suited for preparative purification of complex and high-value compounds, including APIs, pharmaceutical intermediates, peptides, lipids, ionisable lipids, natural products, cannabinoids, alkaloids and flavouring compounds. It is especially useful when high recovery, low adsorption and scalable purification are important.

What is Centrifugal Partition Chromatography?

Centrifugal Partition Chromatography (CPC) is a preparative liquid-liquid chromatography technique. One liquid phase is held inside a rotating rotor by centrifugal force, while a second liquid phase flows through it. Compounds separate according to how they distribute between the two liquid phases.

What is Liquid-Liquid Chromatography?

Liquid-Liquid Chromatography (LLC) is a separation method where both the stationary and mobile phases are liquids. Instead of interacting with silica or another solid material, compounds repeatedly partition between two immiscible liquid phases, allowing them to be separated and collected.

Does LiLiChro offer partnership opportunities?

Yes. LiLiChro works with pharmaceutical companies, CMOs, CDMOs, research teams and industrial partners from feasibility testing through method development and production-scale implementation. Cooperation can include screening studies, equipment installation, training, method development and full industrial projects.

How is it different from HPLC and GC?

The key difference is the stationary phase. Preparative HPLC normally uses a packed solid stationary phase, while CPC uses a liquid stationary phase retained inside a rotor. This eliminates packed-column limitations such as irreversible adsorption, fouling and recurring stationary-phase costs. GC, by contrast, is primarily a gas-phase analytical separation technique for volatile compounds and serves a different purpose.

Is CPC a silica-free separation method?

Yes. CPC does not require silica, C18, resin or another packed solid stationary phase. Separation takes place entirely between two liquid phases. This can reduce consumable costs and the risk of irreversible adsorption of valuable compounds.

How does scale-up from laboratory to production work?

LiLiChro uses the same liquid-liquid chromatography principle across its equipment range. Methods can be developed on miniLiLi, transferred to midiLiLi for gram-scale work, scaled to maxiLiLi for pilot production and finally implemented on prepLiLi for kilogram-scale industrial purification. Flow rate, loading and operating parameters are scaled systematically between systems.